- Available Subtitles: English, Spanish
- Available Audio Tracks: English, English, English (Dolby Digital 2.0 Surround)
- DISCS 1-2: The Feature:
- A new version of the second installment includes 43 minutes of never-before-seen footage incorporated into the film. (approx. 223 minutes)
- Commentary track by writer-director Peter Jackson and writers Fran Walsh and Philippa Boyens
- Commentary track by the design team
- Commentary track by the production/post-production team
- Commentary track by 16 cast members, including Elijah Wood, Sean Astin, Andy Serkis, John Rhys-Davies, Orlando Bloom, Christopher Lee, Bernard Hill, and Miranda Otto
- DISCS 3-4: The Appendices:
- Two discs with hours of original content including multiple documentaries and design/photo galleries with thousands of images to give viewers an in-depth behind-the-scenes look at The Lord of the Rings: The Two Towers
- DISC 3:
- Adapting the book into a screenplay & planning the film
- Designing and inspiration for locations in Middle-earth
- Storyboards to pre-visualization
- Weta Workshop visit: See sculptors in action as they create weapons, armor, creatures, and miniatures from the film
- Atlas of Middle-earth tracing the journey of the Fellowship
- An interactive map of New Zealand highlighting the location scouting process
- Galleries of art and slideshows with commentaries by the artists
- DISC 4:
- Sending the actors into battle: sword fighting
- Principal photography: Stories from the set
- Digital effects including motion capture and the computer program to create the armies of Orcs
- Bigatures: a close-up look at the miniatures
- Galleries of behind-the-scenes photographs and personal cast photos
- Post-Production: editing it all together
- Sound design demonstration
- DVD-ROM Content: Includes access to exclusive online features