- Available Subtitles: English, Spanish
- Available Audio Tracks: English (Dolby Digital 5.1), Spanish (Dolby Digital 2.0 Surround)
- All 24 episodes on seven discs
- Cast and crew commentary on selected episodes
- Original extended version season premiere
- Deleted scenes and alternate takes
- Season 4 teaser and promo
- 24: On the Loose
- Boys and Their Toys
- Biothreat: Beyond the Series
- Multi-angle study
- 45 deleted scenes with optional commentary by Jon Cassar

Features the following episodes:
- 1:00 P.M. - 2:00 P.M. (10/28/2003)
- 2:00 P.M. - 3:00 P.M. (11/4/2003)
- 3:00 P.M. - 4:00 P.M. (11/11/2003)
- 4:00 P.M. - 5:00 P.M. (11/18/2003)
- 5:00 P.M. - 6:00 P.M. (11/25/2003)
- 6:00 P.M. - 7:00 P.M. (12/2/2003)
- 7:00 P.M. - 8:00 P.M. (12/9/2003)
- 8:00 P.M. - 9:00 P.M. (12/16/2003)
- 9:00 P.M. - 10:00 P.M. (1/6/2004)
- 10:00 P.M. - 11:00 P.M. (1/13/2004)
- 11:00 P.M. - 12:00 Midnight (1/27/2004)
- 12:00 Midnight - 1:00 A.M. (2/3/2004)
- 1:00 A.M. - 2:00 A.M. (2/10/2004)
- 2:00 A.M. - 3:00 A.M. (2/17/2004)
- 3:00 A.M. - 4:00 A.M. (2/24/2004)
- 4:00 A.M. - 5:00 A.M. (3/30/2004)
- 5:00 A.M. - 6:00 A.M. (4/6/2004)
- 6:00 A.M. - 7:00 A.M. (4/18/2004)
- 7:00 A.M. - 8:00 A.M. (4/20/2004)
- 8:00 A.M. - 9:00 A.M. (4/27/2004)
- 9:00 A.M. - 10:00 A.M. (5/4/2004)
- 10:00 A.M. - 11:00 A.M. (5/11/2004)
- 11:00 A.M. - 12:00 Noon (5/18/2004)
- 12:00 Noon - 1:00 P.M. (5/25/2004)